yet another sem and yet another paper ppt....though my preparations ...hardly any preps...i m in love with synthetic bio these days...so here it is...abstract to my paper...no sorry abstract to the paper i selected...
A Tunable Synthetic Mammalian Oscillator
Marcel Tigges, Tatiana T. Marquez-Lago, Jo¨rg Stelling & Martin Fussenegger
Nature, Vol. 457,309-312 (15 January 2009)
Abstract
Oscillator circuits mediating the periodic induction of specific target genes are time-keeping devices found in circadian clocks. Here, is described the first controllable mammalian oscillator, constructed based on an auto-regulated sense–antisense transcription control circuit. The circuit encodes a positive and a time delayed negative feedback loop, enabling autonomous, self sustained and tunable oscillatory gene expression. The designed system was monitored using oscillating concentrations of green fluorescent protein with tunable frequency and amplitude, by time-lapse microscopy in real time in individual Chinese hamster ovary cells. This synthetic mammalian clock may provide insight into dynamics of natural periodic processes and also enable complex gene therapy treatments by automating physiological processes in future gene and cell therapies.
References-
1. Jesse Stricker, Scott Cookson (2008) A fast, robust and tunable synthetic gene oscillator. Nature, Vol. 456, 516-520
2. Crosthwaite, S. K. (2004) Circadian clocks and natural antisense RNA. FEBS Lett. 567, 49–54
3. Gossen, M. & Bujard, H. (1992) Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc. Natl Acad. Sci. USA 89, 5547–5551
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